The results indicated that, the hot water extracts for different caffeine. The ability of reo to donate an electron and scavenge the stable 1,1diphenyl2picrylhydrazyl dpph radical was investigated. Comparative study of phenolic compounds and antioxidant. Antioxidant activity and mechanism of protocatechuic acid in vitro background. Hatbased methods measure the classical ability of an antioxidant to quench free radicals by hydrogen donation ah any h donor hence, many scientists feel these are most relevant to reactions where antioxidants typically act. The total antioxidant activity of the sample extracts ranges from 4,400 to 8,800. Antioxidant activity and total phenolics of propolis from. The therapeutic benefit of the medicinal plants is often attributed to their antioxidant properties. Eleucine indica possesses antioxidant, antibacterial and. Oxidation is a chemi cal reaction that transfers electron or hydrogen. Full text pdf journal of applied pharmaceutical science. Nov 20, 2011 recently, there has been growing interest in research into the role of plantderived antioxidants in food and human health. The objective of the present study were to determine the antioxidant activity, total phenolic content, reducing power activity, hydroxyl group reducing activity, estimation of ascorbic acid.
In dpph radical scavenging method the free radicals, 2, 2 diphenyl 1 picrylhydroazyl dpph was used to find antioxidant scavenging activity of. Piper nigrum, piper cubeba, phytochemical, antioxidant activity, dpph. Understanding oxidative stress and antioxidant functions. Antioxidant activity and mechanism of protocatechuic acid in. Photosynthesis is an important source of cellular oxidants foyer and noctor, 2003. Antioxidant activity of extracts from foxtail millet. Rosemary essential oil has already being used as a preservative in food industry due to its antioxidant and antimicrobial activities, but it was shown to possess additional health benefits.
Nutritional composition, mineral content, antioxidant activity and. Antioxidant activity of rosemary rosmarinus officinalis l. The antioxidant activity of various parts of koshu grape was examined for possible utilization. The chemistry behind antioxidant capacity assays journal. Antioxidant activity of the rosemary essential oil reo was evaluated as free radical scavenging capacity rsc. In the present study we carried out a record of the relative antioxidant activity to secondary metabolites content phenol, flavonoid and anthocyanin of onosma dichroanthum boiss. The antioxidant activity of the ethanol extracts of koshu grape stem, leaf, shoot, and skin was determined by measuring 1,1diphenyl2picrylhydrazyl dpph radical scavenging activity and performing the crocin bleaching assay. Antioxidant activity and free radical scavenging capacity. Our results suggest that a combination of all these herbs with green tea can synergistically enhance antioxidant activity and thus lower doses of each herb. Extracts article pdf available january 2011 with 7,475 reads how we measure reads. Phenolic compounds and antioxidant activity of rice braz. Antioxidant activity was determined by spectrophotometric methods using dpph free radical, nitric oxide radical inhibition and ferric reducing antioxidant power assays. Therefore, the aim of this study was to screen the hexane, dicholoromethane, ethyl acetate ea and methanol extracts.
Determination of flavonoid content and antioxidant activity from ferns by ultrasonic extraction jutarat tawekijpokai, jindarat pimsamarn department of chemical engineering, faculty of engineering, king mongkuts university of technology thonburi, bangkok, 10140, thailand email. Phenolic compounds and antioxidant activity of rice. Antioxidant activity and mechanism of protocatechuic acid. Dec, 2001 we extracted polyphenols from carob ceratonia siliqua l. This study also shows the calyx part had strong antioxidant activity. Determination of total phenolic contents the total phenolic content of corn silk extract was determined using the folinciocalteu colorimetric method and gallic acid was used as a standard liu et al. Relationships between phytochemicals and antioxidant activity. Hence, it could therefore be used in pharmacological or food industry as a natural antioxidant. Antioxidant activity of carrageenans has been determined by various methods such as reducing power, iron ion chelation, and total antioxidant activity using betacarotenelinoleic acid system, 1,1diphenyl2picrylhydrazyl radical scavenging, lipid peroxide inhibition, ferric reducing antioxidant power, nitric oxide scavenging, abts radical scavenging, superoxide radical, and hydroxyl radical scavenging assays sokolova et al. View the article pdf and any associated supplements and figures for a. The condensed tannin content determined by the vanillin and proanthocyanidin assay systems was 4. The antioxidant capacity for methanol extract determined in present study was higher than that reported by gholivand et al. Synergistic antioxidant activity of green tea with some herbs. It was observed that all the samples tested had aa, although results varied considerably between extracts, i.
For this purpose, the most commonly methods used in vitro. This study evaluated the antioxidant activity as well as bioflavonoid content of the methanol and ethanolwater extracts of the fresh and dried leaves of tetracarpidium conophorum. Boiling caused lesser antioxidant loss in cooking than did microwaving. Extraction and determination of antioxidant activity of withania somnifera dunal abdul qaiyum ansari 1, syed abrar ahmed1, m. Even under optimal conditions, the calculated rate of h 2 o 2 formation by the pet chain in the chloroplasts during photosynthesis in c 3 leaves is nearly as high 4. Heating dry ginger and turmeric and their essential oils at 120 c results in different degrees of retention of antioxidant activity tiwari and others 2006. Determination of flavonoid content and antioxidant activity. Aqueous methanol, ethanol or water is commonly used for the extraction of many bioactive constituents. Understanding oxidative stress and antioxidant functions to. Evaluation of in vitro antioxidant activity and total phenol content of essential oil. Antioxidant activity of phenolic acids and their metabolites. Antioxidant activity of various part of koshu grape. The antioxidant activity of tested compounds was determined by measuring the percent of dpphi neutralization by them and compared to the standard antioxidants vitamin c and trolox.
The total antioxidant activities of methanol and acetone extract of the samples are presented in table 2. Crocin as a watersoluble carotenoid can be effectively isolated from saffron stigma using the solventassisted extraction methods fig. Antioxidant activity is defined as an limitation of the oxidation of proteins, lipids, dna or other molecules that occurs by blocking the propagation stage in oxidative chain reactions and primary antioxidants directly scavenge free radicals, while secondary antioxidants indirectly prevent the formation of free radicals through fentons reaction huang et al. The total antioxidant free radical scavenging activity of fruits is mainly attributed to the additive and synergistic effects of inherent phytochemicals notably, the phenolic compounds liu, 2003. Triphala used for biliary disorders, diarrhea, dyspepsia, cough. Dpph radical scavenging activity expressed by ic50 was 2. The roots were collected in kiasar mountain 1800 m in mazandaran province, then dried and different extracts were obtained by acetone, ethanol and methanol absolute and. The antioxidant potential of the extract d was comparable to that of vitamin e, and about 1. The beneficial influence of many foodstuffs and beverages including fruits, vegetables, tea, coffee, and cacao on human health has been recently recognized to originate from their antioxidant activity. Comparative antioxidant activity study of some edible plants. Antioxidant is a molecule that inhibits the oxi dation of other molecules. Comparing to trolox, pca shows much more effective antioxidant activity in vitro in both lipid and aqueous media. Antioxidant activities of grape seed extract ranged from 66.
The antioxidant activity of water extracts cold and hot of six caffeine products were carried out. Evaluation of chemical constituents and antioxidant activity. Oct 10, 2011 the antioxidant activity of the essential oils was determined by an accelerated oxidation test in canola oil. Key laboratory of stress physiology and molecular biology for tree fruits of beijing, beijing 100193, pr china. Journal of agricultural and food chemistry 2012, 60 50, 1231212323. In this study, the antioxidant and antimicrobial activities, total phenolic content, and essential oil composition of echinophora tenuifolia l. During different harvesting times from june to september 2011, total phenolic content, total flavonoid content, and antioxidant capacity varied between. In vitro antioxidant activity for individual drug and its combination was determined by 1,1diphenyl2picrylhydrazyl dpph, superoxide, and nitric oxide free radical scavenging methods. The antioxidant activity of investigated essential oil was assessed by abts and dpph assays.
Standardized methods for the determination of antioxidant. In addition to their antioxidant activity, phytochemicals also impart other peculiar beneficial. We extracted polyphenols from carob ceratonia siliqua l. Superoxide radical scavenging activity data represents, mean sem, for n3 figure 3. Methods for total antioxidant activity determination longdom. Pisoschi am, negulescu gp 2011 methods for total antioxidant activity determination. Synthesis and antioxidant properties of the sulfate derivatives of ferulic and caffeic acids and of the acyl glucuronide of ferulic acid. Antioxidant activity and phenolic content of extracts from different pterospartum tridentatum populations growing in portugal. Stankovic department of biology and ecology, faculty of science, university of kragujevac, radoja domanovica 12, 34 000 kragujevac, republic of serbia. Pdf total phenolic content, flavonoid concentration and. Sep 20, 2011 evaluation of chemical constituents and antioxidant activity of sweet.
Relationships between phytochemicals and antioxidant. Inhibition of microsomal peroxidation data represents, mean sem, for n3 the reducing power of different commercial extracts was performed and showed. View antioxidant activity research papers on academia. The purpose of this study was to evaluate the antioxidant activity aa of 19 propolis extracts prepared in different solvent ethanol and propylene glycol. Antioxidant activity, total phenolic and total flavonoid.
Methanol extract of cloves showed the highest antioxidant activity by dpph of. Department of agriculture, arkansas childrens nutrition center, 1120 marshall street. The use of evidencebased complementary and alternative medicine is increasing rapidly. Therefore, the aim of this study was to screen the hexane, dicholoromethane, ethyl acetate ea and methanol. Antioxidant activity, total phenols and phytochemical. Determination of flavonoid content and antioxidant.
Iacopini and others 2008 assessed the antioxidant activity of the extracts and pure compounds using 2 different in vitro tests. Pca was reported to possess various pharmacological effects which may be closely correlated with its antioxidant activities. Total phenolic content, flavonoid concentration and antioxidant activity of marrubium peregrinum l. The aim of this study was to screen various solvent extracts of whole plant of torilis leptophylla to display potent antioxidant activity in vitro and in vivo, total phenolic and flavonoid contents in order to find possible sources for future novel antioxidants in food and pharmaceutical formulations. Antioxidant activity of essential oil of oregano species from. Evaluation of antioxidant activity of tetracarpidium. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements ronald l. Evaluation of antioxidant activity of medicinal plant 885 figure 2. The oregano species showed differences in their chemical composition, radical scavenging activity and antioxidant activity.
Protocatechuic acid pca is a natural phenolic acid widely distributed in plants and is considered as an active component of some traditional chinese herbal medicines such as cibotium barometz l. Eleucine indica ei is traditionally used in ailments associated with liver and kidneys. Antioxidant activity of polyphenols in carob pods journal. Antioxidant activity of oregano, parsley, and olive mill wastewaters in bulk oils and oilinwater emulsions enriched in fish oil. Pdf methods for determining the antioxidant activity. Antioxidant activity of different parts of eggplant.
Comparative study of phenolic compounds and antioxidant activity in different species of cherries yun liu authors y. However, the antioxidant producing capacity of the animal cells are limited halvorsen et. Mar 11, 2014 potential of crocus sativus saffron and its constituent, crocin, as hypolipidemic and antioxidant in rats. Extraction and determination of antioxidant activity of. Antioxidant activity of essential oil of oregano species. Effect of the use of water and different organic solvents such as ethanol or methanol at various concentrations on the total. A detailed study was performed on the antioxidant activity of the methanol extract of whole. For corn bran fractions, the direct method may be suitable for free phenolic content and antioxidant activity, while the sequential method may be proper for bound phenolic content and antioxidant activity. The oregano species showed differences in their chemical composition, radical. Evaluation of chemical constituents and antioxidant. Evaluation of antioxidant activities of bioactive compounds. Similar free antioxidant activities were observed for directed extraction and double extraction. Antioxidant contents increased for all steamed vegetables over that of raw vegetables. Antioxidant activity of rosemary rosmarinus officinalis.
However, the term antioxidant comprises rather complex features. Pdf antioxidant activity and phenolic content of extracts from. Phenolic compounds found in plants were likely to be the main contributors of antioxidant activity of the leaves extracts, though it appears that antioxidant molecules such as xanthones, carotenoids, and chlorophyll. This is an openaccess article distributed under the. High antioxidant activity was found in piper cubeba ethanol extract i.
As antioxidant activity is the basic factor of food function and also plays an important role in herbal medicine function, the antioxidant activity of many herbal resources and kampo formulas has been studied and much information has accumulated. Antioxidant properties, proximate content and cytotoxic. The major components of turmeric oil responsible for this antioxidant activity are and turmerone, curlone, and terpineol carolina and others 2003. Antioxidant activity and free radicalscavenging capacity of gynura. Evaluation of antioxidant activity of medicinal plant. The total polyphenol content in cpp determined by the folin. Journal of agricultural and food chemistry 2008, 56 16, 71517159. Antioxidant activity an overview sciencedirect topics. Resultsphytochemical analysis revealed the presence of tannins, flavonoids, steroids and alkaloids. Natural antioxidant products are increasingly being used to treat various pathological liver conditions considering the role of oxidative stress in their pathogenesis. International journal of pharmtech research coden usa. Stankovic department of biology and ecology, faculty of science, university of kragujevac. Antioxidant activity of tested flavonoids was measured using the stable radical chromogen 2,2diphenyl1picrylhydrazide photometric assay 9, 10. Pisoschi am, negulescu gp 2011 methods for total antioxidant activity.
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